What is humanin?
Before any humanin peptide dosage math, a quick look at the molecule. Humanin is a short peptide encoded within the mitochondrial genome, in the region that carries a gene for a mitochondrial ribosomal RNA. It was first described in 2001 by researchers in Japan who were screening for factors that protected nerve cells in laboratory models related to Alzheimer’s disease. It is considered the first of a group now called mitochondrial-derived peptides, which also includes MOTS-c.
Research vials are also sold as analogs, most commonly HNG, in which one amino acid is swapped; HNG has been reported to be more potent than native humanin in laboratory assays. Check which one your label names, because the calculator works on the mg printed on the vial and cannot tell the difference. Humanin is sold as freeze-dried powder, most often in 5 mg and 10 mg vials.
Why humanin draws research interest
Interest in humanin peptide benefits comes from cell and animal studies examining cell survival under stress, metabolic signaling and ageing, along with observational work measuring circulating humanin levels in people. These are research questions, not established effects, and nothing on this page suggests humanin does anything in a person. For other research-labeled compounds, see the peptide library.
How much BAC water for a humanin vial?
The BAC water you add only sets the concentration. Less water makes a stronger solution, so the same amount fills fewer units; more water makes a weaker one and spreads the same amount over more units. The chart below shows mcg per unit for 5 mg and 10 mg humanin vials across several water volumes.
| BAC water | 5 mg vial | 10 mg vial |
|---|---|---|
| 1 mL | 50 mcg/unit 5 mg/mL | 100 mcg/unit 10 mg/mL |
| 2 mL | 25 mcg/unit 2.5 mg/mL | 50 mcg/unit 5 mg/mL |
| 3 mL | 16.67 mcg/unit 1.667 mg/mL | 33.33 mcg/unit 3.333 mg/mL |
| 5 mL | 10 mcg/unit 1 mg/mL | 20 mcg/unit 2 mg/mL |
Each cell shows mcg per unit on a U-100 syringe (100 units = 1 mL) and the resulting concentration.
A humanin 10mg vial at a given water volume is simply twice as concentrated as a 5 mg vial at the same volume, which the chart makes easy to see. For the reasoning behind these ratios, the BAC water to peptide ratio guide covers it in plain terms.
Worked example: humanin 10mg vial
For example, if the amount you already have is 500 mcg and a 10 mg vial is mixed with 2 mL of BAC water:
The math: 10 mg ÷ 2 mL = 5 mg/mL (5,000 mcg/mL), which is 50 mcg per unit. 500 mcg ÷ 5,000 mcg/mL = 0.1 mL = 10 units on a U-100 syringe.
Three steps sit behind that sentence. Divide the vial amount by the water volume for mg per mL. Convert the amount you have to the same unit (1 mg is 1,000 mcg) and divide it by the concentration for mL. Multiply mL by 100 for U-100 syringe units. Syringe capacity does not change the result: units are the same size on every U-100 syringe, so the mark reads the same on a small or large barrel. The units to mL chart converts in the other direction too.
For a 5 mg vial the steps are identical. For example, if the amount you already have is 250 mcg and the vial is mixed with 2 mL:
The math: 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL), which is 25 mcg per unit. 250 mcg ÷ 2,500 mcg/mL = 0.1 mL = 10 units on a U-100 syringe.
Humanin peptide dosage calculator: what the numbers mean
Searches for humanin peptide dosage usually come from people with a figure in hand who want to see it as syringe units. That conversion is the whole job of this humanin peptide dosage calculator. You enter the mg on the vial, the mL of water and the amount you already have; it returns concentration, volume and units. It does not choose an amount, a schedule or a route, and it cannot say whether any number is appropriate. Research on humanin has been largely preclinical, and there is no approved human product to compare against.
If a result looks off, such as a fraction of a unit or more than a whole syringe, recheck whether the amount was entered as mg or mcg. The mg to mcg conversion guide helps with that check.
Humanin reconstitution notes from supplier labeling
Most research suppliers list bacteriostatic water as the diluent for humanin and its analogs. Some suppliers specify adding the water slowly against the side of the vial and swirling rather than shaking, and many labels call for refrigeration after reconstitution and protection from light. Analogs such as HNG have a slightly different molecular weight from native humanin, which matters for research that works in molar units but not for this mg-based calculator. These are labeling points, not instructions from this site; the product label and a qualified professional take precedence.
Once a multi-dose vial of BAC water is punctured, it should be dated and discarded within 28 days unless the manufacturer gives a different date. See BAC water storage and shelf life for the details.
Regulatory status of humanin
Humanin is a research peptide and is not an FDA-approved drug. Research-labeled vials sold online are not approved products, and a correct calculation cannot confirm identity, purity, sterility or suitability for any use. Other peptides studied in ageing-related laboratory work, such as Epithalon, have their own calculators on this site.
Humanin FAQ
How much BAC water for a 10 mg humanin vial?
There is no single required volume. The water only sets the concentration, and the chart shows mcg per unit for several volumes. Follow the product labeling for the diluent and volume.
Is this a humanin peptide dosage chart?
It is a conversion chart. It converts an amount you already have into mL and U-100 syringe units and does not recommend a dose, schedule or route.
What are humanin peptide benefits?
Humanin is being studied in cell and animal research on cell survival, metabolism and ageing. These are open research questions, not established effects in people.
What is the difference between humanin and HNG?
HNG is an analog of humanin with one amino acid changed. The calculator works the same for both because it uses the mg printed on the vial.
Is humanin FDA-approved?
No. It is sold as a research peptide, and research-labeled vials are not FDA-approved products.