What is TB-500 (thymosin beta-4)?
Most people searching for TB 500 dosage want to turn a number into syringe marks, and that math is what this page covers. TB-500 is a synthetic research peptide sold in connection with thymosin beta-4, a naturally occurring protein of 43 amino acids found in many tissues. Vendors use the two names loosely: some labels say TB-500, some say thymosin beta-4, and some use both. They are not guaranteed to be the same molecule, so the wording on your own vial is what counts.
TB-500 is often discussed alongside BPC-157, and the two are frequently sold in the same catalog or even the same vial. Nothing here describes what TB-500 does in the body. It is sold as a freeze-dried powder, most often in 2 mg, 5 mg and 10 mg vials, and the page focuses on how that powder, BAC water and a U-100 syringe relate.
How much BAC water for a TB-500 vial?
There is no single correct volume. The bacteriostatic water you add only sets the concentration: less water gives a stronger solution and fewer units for the same amount, more water gives a weaker solution and more units. The chart below shows mcg per unit for the three common vial sizes across several water volumes.
| BAC water | 2 mg vial | 5 mg vial | 10 mg vial |
|---|---|---|---|
| 1 mL | 20 mcg/unit 2 mg/mL | 50 mcg/unit 5 mg/mL | 100 mcg/unit 10 mg/mL |
| 2 mL | 10 mcg/unit 1 mg/mL | 25 mcg/unit 2.5 mg/mL | 50 mcg/unit 5 mg/mL |
| 3 mL | 6.67 mcg/unit 0.667 mg/mL | 16.67 mcg/unit 1.667 mg/mL | 33.33 mcg/unit 3.333 mg/mL |
| 5 mL | 4 mcg/unit 0.4 mg/mL | 10 mcg/unit 1 mg/mL | 20 mcg/unit 2 mg/mL |
Each cell shows mcg per unit on a U-100 syringe (100 units = 1 mL) and the resulting concentration.
Read across a row to compare vial sizes at the same water volume, or down a column to see how a tb 500 5mg or tb 500 10mg vial changes as water is added. Each cell is vial mg × 1,000 ÷ (water mL × 100). The BAC water to peptide ratio guide explains why the numbers move the way they do.
Worked example: TB-500 5mg and 10mg in syringe units
Here is the arithmetic for a single vial. For example, if the amount you already have is 500 mcg and a 5 mg TB-500 vial is mixed with 2 mL of BAC water:
The math: 5 mg ÷ 2 mL = 2.5 mg/mL (2,500 mcg/mL), which is 25 mcg per unit. 500 mcg ÷ 2,500 mcg/mL = 0.2 mL = 20 units on a U-100 syringe.
The same method works for the larger vial. For example, if the amount you already have is 1 mg and a 10 mg vial is mixed with 2 mL:
The math: 10 mg ÷ 2 mL = 5 mg/mL (5,000 mcg/mL), which is 50 mcg per unit. 1 mg ÷ 5,000 mcg/mL = 0.2 mL = 20 units on a U-100 syringe.
The steps never change. Divide the vial amount by the water volume to get concentration, divide the amount you have by that concentration to get mL, then multiply by 100 for units, because 100 units equal 1 mL on a U-100 syringe. Put everything in the same unit first: 1 mg is 1,000 mcg. The mg to mcg conversion guide has a quick reference if you need it.
TB 500 dosage calculator: what the numbers mean
A tb-500 calculator, and the TB 500 dosage chart above, answer one question: how many units on a U-100 syringe correspond to an amount you already have, given your vial and your water volume. The calculator takes the mg on the label, the mL of BAC water and the amount you enter, and returns a concentration, a volume and a unit mark.
It does not choose the amount, how often anything is taken, or whether a product is suitable or genuine. That belongs to the product labeling and a qualified professional. A TB 500 dosage chart built this way is a conversion table, not a protocol. If a result looks wrong, such as a fraction of a unit or more than a full syringe, the usual cause is an mg and mcg mix-up or a typo in the vial size; the guide to checking calculator math shows how to verify a result by hand.
BPC 157 and TB 500 in one vial
Some suppliers sell BPC-157 and TB-500 together in a single vial, and TB-500 is also one of the three components in the glow peptide blend. In any blend, every unit you draw carries all the components in the ratio printed on the label. You cannot draw one without the others.
That means the calculator has to work on one component at a time. Enter the mg of TB-500 listed on the blend label as the vial size, not the vial’s total mg, and the result applies to TB-500 only. Repeat with the BPC-157 mg to see the other component at the same syringe mark.
TB-500 reconstitution notes and status
Some suppliers specify bacteriostatic water as the diluent and list a suggested volume on the label or product page; others leave it open. Some suppliers advise letting the water run down the inside of the vial rather than onto the powder. Mixing technique, handling and storage after reconstitution should follow the product labeling; the peptide reconstitution guide explains the general steps.
TB-500 is not an FDA-approved drug, and vials sold online are labeled for research use only. Because TB-500 and thymosin beta-4 wording is used inconsistently, a correct calculation cannot confirm identity, purity or sterility. The numbers are only as good as the label they start from.
TB-500 (Thymosin Beta-4) FAQ
How much BAC water do you add to a 5 mg TB-500 vial?
There is no single required volume. The water only sets the concentration. The chart on this page shows mcg per unit for several volumes; the diluent and volume come from the product labeling.
Is TB-500 the same as thymosin beta-4?
The names are used loosely. Thymosin beta-4 is a naturally occurring 43-amino-acid protein, while TB-500 is a synthetic research peptide sold in connection with it. Check the wording on your own vial.
Is this a TB 500 dosage calculator?
It converts an amount you already have into mL and U-100 syringe units for your vial and water volume. It does not recommend a dose, schedule or treatment.
How do I use the calculator for a BPC-157 and TB-500 blend?
Enter the mg of the one component your amount refers to as the vial size, not the vial’s total. Each unit drawn contains both components in the label ratio.
Is TB-500 FDA-approved?
No. TB-500 is not an FDA-approved drug, and vials sold online are labeled for research use only.